Access to imaging technologies and expertise in the National Microscopy Infrastructure is provided by the four NMI Nodes. The Nodes offer a powerful portfolio of state-of-the-art technologies in the fields of biological imaging. Each Node brings in a different combination of technologies as well as their expertise with distinct applications and methods.
Multimodal microscopy
Multimodal assimilation of technologies provides complementary and often unique information, spatial, structural, biochemical and biophysical details from the same target sample.
Super-resolution structured illumination microscopy (SIM)
SIM microscopy system can produce twice the resolution of conventional optical microscopes
Single molecule localization microscopy
Within the NMI infrastructure we have the equipment and experience to perform single molecule localization microscopy with both PALM and STORM.
Stimulated emission depletion microscopy (STED)
At the NMI infrastructure we have the equipment and experience to perform STED in a diversity of sample preparations.
Light sheet microscopy
Permits optical sectioning of large samples, with little to no phototoxicity or photobleaching and high temporal resolution.
Lattice light sheet microscopy
Volumetric imaging with high resolution, minimal exposure and phototoxicity, 4D over very long times.
Correlative light electron microscopy (CLEM)
Equipment and experience to perform CLEM using transmission electron microscopy (TEM) and scanning electon microscopy (SEM).
Correlative light and atomic force microscopy (CLM-AFM)
Equipment and experience to perform CLM-AFM.
Cryo-electron microscopy (Cryo-EM)
Equipment and experience to perform Cryo EM.
Fluorescence correlation spectroscopy (FCS/FCCS)
Measures diffusion coefficients and concentrations of fluorescent molecules.
Intravital microscopy (IVM)
NMI offers a broad range of intravital microscopy applications with multi- and/or single-photon microscopy and FLIM.
BioImage analysis
Support and training in state-of-the-art image analysis.