Access to imaging technologies and expertise in the National Microscopy Infrastructure is provided by the five NMI Nodes. The Nodes offer a powerful portfolio of different state-of-the-art technologies in the fields of biological imaging. Each Node brings in a different combination of technologies as well as their expertise with distinct applications and methods.
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Multimodal microscopy
Multimodal assimilation of technologies provides complementary and often unique information, spatial, structural, biochemical and biophysical details from the same target sample.
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Super-resolution Structured illumination microscopy (SIM)
SIM microscopy system can produce twice the resolution of conventional optical microscopes
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Single molecule localization microsopy
Within the NMI infrastructure we have the equipment and experience to performe single molecule localization microscopy with both PALM and STORM.
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STED – Stimulated emission depletion microscopy
At the NMI infrastructure we have the equipment and experience to perform STED in a diversity of sample preparations.
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Light sheet microscopy
Permits optical sectioning of large samples, with little to no phototoxicity or photobleaching and high temporal resolution.
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Lattice Light sheet microscopy
Volumetric imaging with high resolution, minimal exposure and phototoxicity, 4D over very long times.
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CLEM – Correlative light electron microscopy
Equipment and experience to perform CLEM using transmission electron microscopy (TEM) and scanning electon microscopy (SEM)
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CLM-AFM – Correlative Light and Atomic Force Microscopy
Equipment and experience to perform CLM-AFM
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Cryo electron microscopy
Equipment and experience to perform Cryo EM.
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FCS/FCCS – Fluorescence correlation spectroscopy
Measures diffusion coefficients and concentrations of fluorescent molecules.
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IVM – Intravital microscopy
NMI offer a broad range of intravital microscopy applications with multi- and/or single-photon microscopy and FLIM.
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BioImage Analysis
Support and training in state-of-the-art image analysis.